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KMID : 0624620100430010040
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BMB Reports
2010 Volume.43 No. 1 p.40 ~ p.45
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Transduced PEP-1-AMPK inhibits the LPS-induced expression of COX-2 and iNOS in Raw264.7 cells
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Shin Min-Jea
Cho Yong-Jun
Choi Soo-Young
Lee Kwang-Gill
Park Jin-Seu
Kim Dae-Won
An Jae-Jin
Jang Sang-Ho
Eum Won-Sik
Lee Hae-Ran
Kang Seok-Woo
Lee Yeom-Pyo
Kweon Hae-Yong
Cho Sung-Min
Sheen Seung-Hoon
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Abstract
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AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases.
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KEYWORD
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AMPK, Cyclooxygenase-2 (COX-2), Inducible nitric oxide synthase (iNOS), Lipopolysaccaride (LPS), Protein transduction
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